2 × Medino® AdvanceFast PCR Master Mix

Product name2 × Medino® AdvanceFast PCR Master Mix

 

Cat.No.LM201001-1
Size1 mL/5×1 mL
Storage and shippingThe product is shipped with dryice and can be stored at -25℃ ~ – 15 ℃ for  1 year.

Description

Product description
2× Medino®  AdvanceFast PCR Master Mix is a ready-to-use 2× pre-mixed solution containing  Medino®  AdvanceFast  High-Fidelity  DNA  Polymerase,  dNTPs,  and  an  optimized buffer  system,  which  contains  preadded  electrophoresis  indicators.  The  pre-mix  contains pre- added electrophoresis indicator, PCR products can be directly electrophoresed, the amplification products are flat ends. 2× Medino® AdvanceFast PCR Master Mix  has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with  only  the  primers  and  templates.  In  addition,  the  product  also  contains  a  specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.
Instructions
  1. Recommended PCR Reaction System
ComponentsVolume μLFinal concentration
2 × Medino® AdvanceFast PCR Master Mix251 ×
Templateb)x
Forward Primer (10 μmol/L)c)20.4 μmol/L
Reverse Primer (10 μmol/L)20.4 μmol/L
ddH2OUp to 50
[Note]:
  1. In 1× premixes containing 2 mM Mg2+ and 200 μM dNTPs.
  2. b) Recommended range 10-200 ng, cDNA sample upload volume range not more than 1/10 of the reaction system, recommended 1-2.5 μL.
  3. c) The final primer concentration in the PCR reaction system ranges from 2-1 μM, and 0.4 μM is recommended.
2.Reaction program
Cycle stepTemp.TimeCycles
Initial denaturation98 °C30 sec1
Denaturation98 °C10 sec30-35
Annealinga)60 °C5 sec
Extensionb)72 °C5-10 sec/kb
Final extension72 °C2 min1
[Note]:
  1. Recommended temperature: 60°C, a temperature gradient can be set up to find the optimal temperature for primer The recommended annealing time is set to 5 sec and can be adjusted from 5-30 sec. Too long annealing time may result in diffuse amplification products on the gel.
  2. Extension time: Recommended 5 sec/kb, can also be extended to 10 sec/kb as needed.
Notes
  1. This product is for research use only.
  2. Please operate with lab coats and disposable gloves for your safety.

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