2× Medino® PCR Master Mix (With Dye)

Product2× Medino® PCR Master Mix (With Dye)
Catalog No.LM201003-2
Storage conditionsThis product should be stored at -25 ~ -15℃ for 2 years.

Description

Product description

2× Medino®  PCR Master Mix is a ready-to-use conventional PCR pre-mixed solution containing Medino® Taq DNA Polymerase and dNTP mix Compound, MgCl2, and the optimized buffer system. In the reaction, only primers and templates can be added, greatly simplifying the operation steps of the experiment. The product contains the bromophenol blue dye, and the PCR products can be directly subjected to electrophoresis. This product contains excellent stabilizer and can be placed for 3 months at 4℃. PCR The product has a 3 ′-dA protruding end and can be easily cloned into the T vector.

Components

Cat.No.LM201003-2
Size1 mL/5×1 mL

Instructions

  1. Recommended PCR reaction systems.
ComponentsVolume (μL)Final concentration
2× Medino® PCR Master Mix(With Dye)25
Templatex
Forward Primer 10 μmol/L2
Reverse Primer 10 μmol/L2
ddH2OUp to 50
[Note]:
  1. a) Template usage: genomic DNA: 50 – 200 ng; plasmid DNA:0.1 – 10 ng.
  2. b) Mg2+ concentration: This product contains 3 mM of MgCl2, which is suitable for most PCR reactions.
  1. Reaction program
Cycle stepTemp.TimeCycles
Initial denaturation94℃5min1
Denaturation94℃30 sec35
Annealing50 – 60℃30sec
Extension72℃30 – 60 sec/kb
Final extension72℃10 min1
[Note]:
  1. Annealing temperature: please refer to the theoretical Tm value of primer, the annealing temperature can be set 2 – 5℃ lower than the theoretical value of primer.
  2. Extension time: Recommended 30 sec/kb for molecular identification. Gene cloning suggested 60 sec/kb to ensure the highest amount of product.

Notes

  1. a) For your safety and health, please wear a lab coat and disposable gloves during
  2. b) This product is for research use only!
  3. c) The PCR product of this product is not suitable for polyacrylamide gel electrophoresis.

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