5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactoside)

CAS:                               7240-90-6

Catalog Number:         MBCA003011

Delivery time:              1 Month

Categories: ,
GHGJFKFKJFHJKFJK

Properties

MF:                     C14H15BrClNO6

MW:                   408.63 g/mol

Melting point:  230 °C

Boiling point:    673.9±55.0 °C(Predicted)

Density:             1.5563 (rough estimate)

storage temp.: -20°C

solubility:          soluble in 100 mM in DMSO.

Pka:                    12.74±0.70(Predicted)

Form:                 Powder 

Color:                White

Description

An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.

A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm)

In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.

magenta b-galactosidase substrate

A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).

Hazard Codes:              Xi

Risk Statements:          36/37/38

Safety Statements:       24/25-37/39-26 

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