Disodium 4-nitrophenylphosphate

CAS: 4264-83-9

Artikel Nr: B6010

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Category:

Properties

Synonyms:

Disodium 5-nitrophenylphosphate;Disodium 4-nitrophenylphosphate ,99%;

4-Nitrophenyl phosphate disodium salt hexahydrate p.a.;

p-NPP (p-Nitrophenyl phosphate, disodium, hexahydrate);

PNPP, 2NA;PNP-PHOS DINA;PNPP LIQUID SUBSTRATE SYSTEM;

P-NITROPHENYL PHOSPHATE DISODIUM

CAS: 4264-83-9

MF: C6H4NNa2O6P

MW: 263.05

EINECS: 224-246-5

Product Categories: Enzyme substrates;Substrates;Biological Buffers;Buffer Convenience Packaging;Buffer SolutionsBiological Buffers;Buffers A to Z

Mol File: 4264-83-9.mol

Melting point: >300 °C(lit.)

storage temp: -20°C

solubility: H2O: 1 tablet/3 mL 25 mM, clear, light yellow

form: tablets

color: white to pale yellow

Water Solubility: Miscible with water. Immiscible with ethanol and acetone.

BRN: 5324661

Stability: Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.

InChIKey: KAKKHKRHCKCAGH-UHFFFAOYSA-L

 

 

Safety

Hazard Codes: Xi,Xn

Signal word: Danger

Risk Statements: 36/38-36/37/38-48/22-41-38-21/22

Safety Statements: 26-36-24/25-23-36/37/39

WGK Germany: 3

F: 10-21

TSCA: Yes

Hazard statements: H290-H315-H318-H361fd-H373

Precautionary statements: P202-P234-P280-P302+P352-P305+P351+P338-P308+P313

Uses

PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.

 

General Description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.

 

Biochem/physiol Actions

Primary TargetSubstrate for phosphatase

 

Purification Methods

Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]

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