Isopropyl-beta-D-thiogalactopyranoside

IPTG (Isopropyl-Beta-D-Thiogalactopyranoside)

CAS                              No.367-93-1

Catalog Number:       MBCA003010

Delivery time:            1 Month

Categories: , ,

Properties

Molecular Formula:      C9H18O5S

Molecular Weight:         238.3 g/mol

Melting point:                 105 °C

Boiling point:                  350.9°C (rough estimate)

Density:                           1.3329 (rough estimate)

Storage temp.:               2-8°C

Form:                               Crystalline Powder

Color:                              White

water Solublity             Soluble

Description

General Description

IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.

Uses

Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.

Safty

Hazard statements                H319-H351

Precautionary statements    P281-P305+P351+P338

Hazard Codes                           Xn,Xi

Risk Statements                       19-40-66-36/37/38

Safety Statements                    23-24/25-36/37-22-36/37/39-27-26

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