Medino® Bst Plus DNA Polymerase (40 U/μL)

 

Catalog No.LM702001
Storage conditionsStore at -25~-15℃, valid for 2 year.
Enzyme Storage Buffer10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA,

1 mM DTT, 0.1% Triton X-100, 50% Glycerol

pH 7.5 @ 25°C

Heat Inactivation85°C, 5 min
Unit Definition1 unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitate under the reaction conditions of 65°C for 30 minutes.
Product ApplicationsThis product is suitable for various isothermal amplification reactions such as LAMP, CPA, RCA, etc.
Categories: ,

Product description

Medino® Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5´-3´ exonuclease activity. The enzyme has stronger 5´-3´ DNA polymerase activity, strand displacement activity and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.

CATComponents Name

LM702001-ES92

(8,000 U)

LM702001-ES97

(40,000 U)

LM702001-AMedino® Bst Plus DNA Polymerase (40 U/μL)

200 μL

 

1 mL

 

LM702001-B10× Medino® Bst Plus DNA Polymerase Buffer

1 mL

 

3 × 1 mL

 

LM702001-C100 mM MgSO41 mL3 × 1 mL

Instructions

  1. Reaction System
ComponentsVolume (μL)Final Concentration
10x Reaction Buffer2.51 x
100 mmol/L MgSO40.753 mmol/L+ 2 mmol/L in buffer = 5 mmol/L
  dNTP Mix (25 mmol/L each)1.41.4 mmol/L each
dUTP (25 mmol/L) (optional)1.41.4 mmol/L
UDGase (1U/μl) (optional)1  0.04 U
DNA10 ng – 1μg
10x Primers2.5
Medino® Bst Plus DNA Polymerase (40U/μl)11.6 U/μl
ddH2Oto 25 

[Note]:

  1. The amount of Medino® Bst Plus DNA Polymerase can be adjusted and optimized according to different experiments.
  2. 10× Reaction Buffer: 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Tween-20, pH 8.8 @ 25°C.
  3. The Mg2+ concentration can be adjusted between 4–10 mM according to different experiments.
  4. 10× Primers: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B each.
  • Reaction program
Temp.TimeFunction
25 – 37°C5 – 10 minDegradation of templates containing uracil (optional)
65°C30 – 60 minReaction
85°C5 minInactivation

Notes

The enzyme should be stored in a cooler or on ice during use and immediately placed at -20°C after use.

This product is for research use only.

For your safety and health, please wear a lab coat and disposable gloves during operation.

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