Medino® C15P2 TaqMan Multiplex qPCR Probe Kit

 

Cat.No. LM602008
Size100T/1000T /10000T
Storage and shipping1. Shipping and Storage Instructions: The product is shipped with ice packs.

2. Store at -20°C. The product is stable for 24 months.

Application equipment

 

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

Product description

Medino® C15P2 TaqMan Multiplex qPCR Probe Kit is a fluorescence-based quantitative PCR (qPCR) reagent that utilizes the company’s next-generation antibody-mediated hot-start Taq enzyme. The 2× reaction buffer contains Mg2+ and dNTPs, along with factors that effectively inhibit non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions. This allows for up to fourplex reactions while ensuring primer efficiency.

Components

Components No.Components Name        Specifications
  100T1000T10000T
LM602008-A2×TaqMan qPCR buffer1.25 mL12.5 mL125 mL
LM602008-BHotstart D-Taq(5 U/uL)60 μL600 μL6 mL

Operate

qPCR reaction System

Components Volume μLFinal Conc.
2× TaqMan qPCR buffer12.51 ×
Hotstart D-Taq(5 U/μL)0.60.12 U/μL
Primer mix (10 μM))x0.1 μM-0.5 μM
Probe mix (10 μM)x50 nM-250 nM
DNA/cDNA template1-10
ddH2OUp to 25

[Note]: Primer concentration and DNA amount can be adjusted according to experimental requirements.

Reaction program

Cycle stepTemp.TimeCycles
Initial denaturation95 °C5 min1
Denaturation95 °C15 sec45
Annealing/Extension60 °C30 sec

[Note]: Annealing/extension temperature can be adjusted according to experimental requirements.

 

Notes

For your safety and health, please wear lab coats and disposable gloves for operation.

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