Medino® C164P1 Universal TaqMan multiplex qPCR Kit (UDG plus)

 

Cat.No. LM601014
Size100T/1000T /10000T
Storage and shippingStorage Conditions: Store at -25°C to -15°C. Shelf life is 18 months.
Application equipment

 

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne ™ , StepOne Plus ™ , 7500, 7500 Fast, ViiA ™ 7,

QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

Product description

This product allows for multiplex fluorescence quantitative PCR reactions in a single reaction well, accommodating up to four targets. It has been extensively optimized for multiplex reaction buffer systems to enhance amplification efficiency and facilitate effective amplification of low-concentration templates. This product is suitable for gene typing and multiplex gene quantification analysis. 5×Medino® C164P1 Universal TaqMan qPCR buffer requires additional addition of dNTPs, dUTP, Mg2+, Taq enzyme, and UDG enzyme.

Components

Components No.Components NameSpecifications
  100T1000T10000T
LM601014-A5×Medino® C164P1 Universal TaqMan qPCR buffer500 μL5 mL50 mL
LM601014-BE-Taq(20U/μL)10 μL100 μL1 mL
LM601014-C250mM MgSO4125 μL1.25 mL12.5 mL
LM601014-D25mM dNTPs60 μL600 μL6 mL
LM601014-E100mM dUTP10 μL100 μL1 mL
LM601014-FUDG Enzyme (1U/μL)25 μL250 μL2.5 mL

 

Operate

qPCR reaction System

Components No.Components Volume μLFinal Conc.
LM601014-A5×Medino® C164P1 Universal TaqMan qPCR buffer51 ×
LM601014-BE-taq (20U/μL)0.1 
LM601014-C250mM MgSO41.25 
LM601014-D25mM dNTPs0.6 
LM601014-E100mM dUTP0.1 
LM601014-FUDG Enzyme (1U/μL)0.25 
 Primer mix (10 μM)X0.1-0.5 μM
 Probe mix (10 μM)X50-250 nM
 Template DNA/cDNA1-10
 ddH2Oup to 25

Reaction program

Cycle stepTemp.TimeCycles
Contamination Degradation37 °C5 min1
Initial denaturation97 °C30 sec1
Denaturation97 °C3 sec45
Annealing/Extension60 °C15 sec

[Note]: Amplification Reaction: Adjust the amplification reaction temperature according to the Tm value of the designed primers. Fluorescence Signal Acquisition: Different qPCR instruments require different fluorescence signal acquisition times. Please set according to the shortest time limit.

 

Notes

  1. This product is for research use only.
  2. For your safety and health, wear a lab coat and disposable gloves while handling.
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