Medino® C64P1 TaqMan Multiplex qPCR Kit

 

Catalog No.LM602009
Storage conditionsStore at -20°C with a shelf life of 1 year.
Hot StartBuilt-in hot start

Product description

Medino® C15P2 TaqMan Multiplex qPCR Probe Kit is a fluorescence-based quantitative PCR (qPCR) reagent that utilizes the company’s next-generation antibody-mediated hot-start Taq enzyme. The 2× reaction buffer contains Mg2+ and dNTPs, along with factors that effectively inhibit non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions. This allows for up to fourplex reactions while ensuring primer efficiency.

Components

Components No.Components Name        Specifications
  100T1000T10000T
LM602008-A2×TaqMan qPCR buffer1.25 mL12.5 mL125 mL
LM602008-BHotstart D-Taq(5 U/uL)60 μL600 μL6 mL

Operate

qPCR reaction System

Components Volume μLFinal Conc.
2× TaqMan qPCR buffer12.51 ×
Hotstart D-Taq(5 U/μL)0.60.12 U/μL
Primer mix (10 μM))x0.1 μM-0.5 μM
Probe mix (10 μM)x50 nM-250 nM
DNA/cDNA template1-10
ddH2OUp to 25

[Note]: Primer concentration and DNA amount can be adjusted according to experimental requirements.

 

Reaction program

Cycle stepTemp.TimeCycles
Initial denaturation95 °C5 min1
Denaturation95 °C15 sec45
Annealing/Extension60 °C30 sec

[Note]: Annealing/extension temperature can be adjusted according to experimental requirements.

 

Notes

For your safety and health, please wear lab coats and disposable gloves for operation.

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