Medino® C64P1 TaqMan Multiplex qPCR Kit


Catalog No.LM602009
Storage conditionsStore at -20°C with a shelf life of 1 year.
Hot StartBuilt-in hot start

Product description

Medino® C15P2 TaqMan Multiplex qPCR Probe Kit is a fluorescence-based quantitative PCR (qPCR) reagent that utilizes the company’s next-generation antibody-mediated hot-start Taq enzyme. The 2× reaction buffer contains Mg2+ and dNTPs, along with factors that effectively inhibit non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions. This allows for up to fourplex reactions while ensuring primer efficiency.


Components No.Components Name        Specifications
LM602008-A2×TaqMan qPCR buffer1.25 mL12.5 mL125 mL
LM602008-BHotstart D-Taq(5 U/uL)60 μL600 μL6 mL


qPCR reaction System

Components Volume μLFinal Conc.
2× TaqMan qPCR buffer12.51 ×
Hotstart D-Taq(5 U/μL)0.60.12 U/μL
Primer mix (10 μM))x0.1 μM-0.5 μM
Probe mix (10 μM)x50 nM-250 nM
DNA/cDNA template1-10
ddH2OUp to 25

[Note]: Primer concentration and DNA amount can be adjusted according to experimental requirements.


Reaction program

Cycle stepTemp.TimeCycles
Initial denaturation95 °C5 min1
Denaturation95 °C15 sec45
Annealing/Extension60 °C30 sec

[Note]: Annealing/extension temperature can be adjusted according to experimental requirements.



For your safety and health, please wear lab coats and disposable gloves for operation.



There are no reviews yet.

Be the first to review “Medino® C64P1 TaqMan Multiplex qPCR Kit”

Your email address will not be published. Required fields are marked *