Medino® Hotstart Direct Taq DNA Polymerase, 5 U/μL

 

Catalog No.LM203003
Storage conditionsShipment with ice packs. Store at -20°C with a shelf life of 2 years.
Hot StartBuilt-in hot start

 

Product description

Medino® Hotstart Direct Taq DNA Polymerase is a heat-activated DNA polymerase that is tolerant to blood and other inhibitors. This product utilizes antibody blocking and possesses excellent amplification sensitivity and specificity. The antibody is fully deactivated by heating at the denaturation temperature for 30 seconds, releasing the DNA polymerase activity. The use of this hot-start Taq enzyme effectively suppresses non-specific amplification caused by primer misannealing.

 

Components

 Catalog No.SpecificationVolume
Medino® Hotstart Direct Taq DNA Polymerase, 5 U/μLLM203003ES72250 U50 μL
LM203003ES76500 U100 μL
LM203003ES801000 U200 μL
LM203003ES9210 KU1 mL× 2
LM203003ES9325 KU1 mL× 5
LM203003ES9450 KU10 mL

Instructions

1.Reaction System

ComponentsVolume (μL)Final Concentration
2×Buffera251 x
Primer/Probe mixbx0.1 μM-0.5 μM
Hotstart D-Taq (5 U/μL)c1.20.12 U/μL
Template DNAdx0.1-100 ng
ddH2Oup to 50

[Note]:

  1. According to the specific experimental application, it is necessary to prepare the corresponding reaction buffer.
  2. The primer concentrations in the table are recommended concentrations. They can be adjusted according to the specific experimental conditions to achieve the optimal concentrations.
  3. Adjust the amount of Taq enzyme according to the specific experimental application.
  4. The DNA amount in the table are recommended concentrations. They can be adjusted according to the specific experimental conditions to achieve the optimal concentrations.

2.Reaction program

CyclestepTemp.TimeCycles
Initial denaturation95°C5 min1
Denaturation95°C15 s

45

 

Annealing/Extension60°Ca30 sb

[Note]:

  1. Amplification reaction: The amplification reaction temperature should be adjusted based on the Tm value of the designed primers.
  2. Fluorescence signal collection: Different qPCR instruments require different fluorescence signal collection times. Please set the collection time according to the shortest time limit.

Notes

For your safety and health, wear lab coats and disposable gloves during operation.

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