Medino® HotStart E-Taq DNA Polymerase,  (5 U/μL)

ProductMedino® HotStart E-Taq DNA Polymerase,  (5 U/μL)
Catalog No.LM203004
Storage conditionsThis product should be stored at -20℃ for 2 years.


Product description Medino® Hotstart E-Taq DNA Polymerase is a hot-start DNA polymerase with double antibody independently developed by the company. This product closes not only the Taq DNA, the polymerase 5 ′-3 ′ polymerase activity, but also the 5 ′-3 ′ exonuclease activity. Heating the 30 sec blocking antibody at the pre-denaturation temperature was completely inactivated, releasing DNA polymerase activity and exonuclease activity. The double sealing feature can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decrease of fluorescence signal generated by probe degradation, and the double guarantee makes the in vitro detection reagent more stable in transportation or use at room temperature.   Components
Size250 U/500 U/1000 U/10000 U
  1. Recommended PCR reaction systems.
ComponentsVolume (μL)Final concentration
Primer/Probe mixbX0.1 μmol/L-0.5 μmol/L
HotStart E-Taq DNA Polymerase, (5 U/μL)1.20.12 U/μL
ddH2OUp to 50
TemplateX0.1-100 ng
  1. Reaction program
Cycle stepTemp.TimeCycles
Initial denaturation95℃5 min1
Denaturation95℃15 sec  45
Annealing60℃30 sec
Extension60℃30 sec
  1. The amplification reaction temperature was adjusted according to the designed primer Tm value.
  2. Fluorescent signal acquisition: the different qPCR instruments required for the fluorescence signal  acquisition time is different, please set it according to the minimum time limit.
  1. a) For your safety and health, please wear a lab coat and disposable gloves during operation.
  2. b) This product is for research use only!


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