Medino® HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)

ProductMedino® HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)
Catalog No.LM203005
Storage conditionsThis product should be stored at 2~8℃ for 1 years.

Description

Product description

Medino® HotStart E-Taq DNA Polymerase, Glycerol-free is a heat-start DNA polymerase with double sealing by using double antibodies independently developed by the company. This product closes not only the Taq DNA, the polymerase 5 ′- 3 ′ polymerase activity, but also the 5 ′- 3 ′ exonuclease activity. The antibody was completely inclosed by heating 30 sec at the pre-denaturation temperature, releasing DNA polymerase activity and exonuclease activity. The double sealing feature can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decrease of fluorescence signal generated by the probe degradation, and the double guarantee makes the in vitro detection reagent used in transport or at room temperature The process is more stable. This product does not contain glycerol and is specially configured with lyophilized reagents.

Components

Cat.No.LM203005
Size500 U / 1000 U / 10 KU / 50 KU / 100 KU

Instructions

  1. Recommended PCR reaction systems.
ComponentsVolume (μL)Final concentration
2×Buffera25
Primer/Probe mixbX0.1 μmol/L-0.5 μmol/L
HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)1.20.12 U/μL
ddH2OUp to 50
TemplateX0.1-100 ng
  1. Reaction program
Cycle stepTemp.TimeCycles
Initial denaturation95℃5 min1
Denaturation95℃15 sec45
Annealing60℃30 sec
Extension60℃30 sec
[Note]:
  1. The amplification reaction temperature was adjusted according to the designed primer Tm value.
  2. Fluorescent signal acquisition: the different qPCR instruments required for the fluorescence signal  acquisition time is different, please set it according to the minimum time limit.

Notes

  1. a) For your safety and health, please wear a lab coat and disposable gloves during operation.
  2. b) This product is for research use only!

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