Medino® Lyo-Ready I TaqMan Multiplex qPCR Mix

Product nameMedino® Lyo-Ready I TaqMan Multiplex qPCR Mix
Cat.No. LM602011
Size100T/1000T
Storage and shippingStorage Conditions: Store at -25°C to -15°C. Shelf life is 12 months.
Application equipment

 

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™ , StepOne Plus ™ , 7500, 7500 Fast, ViiA™ 7,

QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

 

Description

Product description

The Medino® Lyo-Ready I TaqMan Multiplex qPCR Kit is a lyophilizable reagent developed using our company’s next-generation antibody-based hot-start Taq enzyme technology. This fluorescent quantitative qPCR kit is suitable for dual-labeled probes such as FAM, HEX, TET, JOE, and ROX (also compatible with Molecular Beacon probes). The reagent includes lyophilization protectants, hot-start Fast Taq DNA polymerase, dNTPs, and other components, making it ready for direct lyophilization and use in PCR machines. This product does not contain ROX reference dye, ensuring compatibility with various qPCR instruments.

 

Operate

Lyophilizable System for On-Machine Use

Components No.Components Volume μLFinal Conc.
LM602011Lyo-ReadyTaqMan qPCR Mix201 ×
 Primer Mix (10 μM)*x0.1-0.5 μM
 Probe Mix (10 μM)x50-250 nM
 Total Volume21-25 –

[Note]: * The primer concentration can be adjusted according to experimental requirements. However, the total volume should not exceed 5 μL.

 

Lyophilization Procedure

Cycle stepTemp.Time SettingsDurationVacuum
Pre-Freezing Step-45℃10 min60 min——

 Drying Stage

 

 

-45℃10 min60 min0.01mbar
-40℃10 min600 min0.01mbar
-35℃10 min120 min0.01mbar
-30℃20 min60 min0.01mbar
-20℃20 min60 min0.01mbar
-10℃20 min60 min0.01mbar
0℃20 min60 min0.01mbar
Pressure Rise Test
Drying Stage Analysis5 ℃10 min20 min0.01mbar
10 ℃10 min20 min0.01mbar
15 ℃10 min20 min0.01mbar
20 ℃10 min20 min0.01mbar
25 ℃10 min240 min0.01mbar
Pressure Rise Test

 

ComponentVolume μLFinal Conc.
20 μL system freeze-dried pellet1
Primer Mix (10 μM)x0.1-0.5 μM
Probe Mix (10 μM)x50-250 nM
Template1-25

ddH2O

 

up to 25

The template should be dissolved in ddH2O, and ddH2O should be used as the elution buffer for nucleic acid extraction reagents.

 

Cycle stepTemp.TimeCycle
Initial denaturation95℃5 min1
Denaturation95℃15 sec45
Annealing/Extension60℃30 sec

[Note]: The annealing/extension temperature can be adjusted as needed according to experimental requirements.

 

Notes

For your safety and health, please wear a lab coat and disposable gloves while handling.

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