Medino® Lyophilized Bst Plus DNA Polymerase (60 U/μL, Glycerol-Free)

 

Catalog No.LM702002
PolymeraseBst DNA Polymerase
Storage conditionsStore at -25~-15℃, valid for 1 year. Please avoid light.
Heat Inactivation85°C, 5 min
Unit Definition1 unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitate under the reaction conditions of 65°C for 30 minutes.
Product ApplicationsIsothermal amplification reaction
Categories: ,

Product description

Medino® Bst Plus DNA Polymerase is derived from Bacillus stearothermophilus DNA Polymerase l. The 5 ‘-3’ exonuclease activity was removed by genetic engineering method, but the 5 ‘-3’ DNA polymerase activity and strong chain replacement activity were retained. Compared with wild-type Bst DNA Polymerase, the polymerase showed great improvement in amplification speed, yield, salt tolerance and thermal stability, and increased dUTP tolerance. It was very suitable for isothermal amplification reactions such as LAMP reaction. This product freeze-dried glycerin- free Bst Plus DNA Polymerase to solve the problem of glycerinase-free instability and could be used to make freeze-dried products.

Components

Components No.Components Name

LM702002-ES60

(12 KU)

LM702002-ES97

(120 KU)

LM702002-ES98

(1,200 KU)

LM702002-ALyophilized Bst Plus DNA Polymerase (60 U/μL, Glycerol-Free)

1 Vial

(200 μL)

 

5 Vials

(400 μL)

 

10 Vials

(2 mL)

 

LM702002-BLyophilized monoenzyme redissolution solution

200 μL

 

2 mL

 

20 mL

Instructions

1.Reaction System

ComponentsVolume (μL)Final Concentration
2.5x pH Sensitive Reaction Buffer101 x
1M MgSO40.2 
25 mM d(AUCG)TP1.4 
UDGase (Glycerol-free)0.005 
Reverse Transcriptase (Glycerol-free)0.075 
Lyophilized Bst Plus DNA Polymerase2 
RNase Inhibitor (Glycerol-free)0.2
10x Primers2.51x
Templatex
ddH2OUp to 25

[Note]:

  1. The recommended concentration of 25 mmol/L d(A/U/G/C)TP mix was 1.2 – 1.4mmol/L
  2. 10x Primers: 16 μmol/L FIP/BIP, 2 μmol/L F3/B3, 4 μmol/L Loop F/B each. The ratio of primers also could be adjusted appropriately.

2.Reaction System

The reaction was incubated at 65°C for 30 – 60 min. Heat inactivation was carried at 85°C for 5 min.

If UDG was used in the reaction system, the UDG digestion reaction should be proceeded (such as incubation at 25°C for 10 min).

 

Notes

This product is for research use only.

For your safety and health, please wear a lab coat and disposable gloves during operation.

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