Medino® Superpro I TaqMan qPCR Master Mix(UDG plus)

Cat.No. LM602011
Size100T/500T/1000T /5000T/10000T/40000T
Storage and shippingStore at -25 to -15°C, with a shelf life of 1 year.
Application equipment

 

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

PDF Download: LM602011

 

Product description

Medino® Superpro I TaqMan qPCR Master Mix (UDG plus) is a fluorescent quantitative pre-mixed solution that utilizes the company’s next-generation antibody-based hot-start Taq enzyme. The 2× Mix pre-mixed reagent contains reaction buffer and enzyme mix, which uses dUTP instead of dTTP. Additionally, it includes factors that effectively suppress non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions. This greatly reduces non-specific PCR amplification and contamination, ensuring primer efficiency while allowing for up to fourfold reactions. This product contains UDG enzyme, which effectively prevents the risk of aerosol contamination.

 

Components

Components No.Components Name
LM6020112×TaqMan qPCR Mix

 

Instructions for Use

qPCR reaction System

Components Volume μLFinal Conc.
2× TaqMan qPCR Mix12.51 ×
Primer mix (10 μM))x0.1 μM-0.5 μM
Probe mix (10 μM)x50 nM-250 nM
DNA/cDNA template1-10
ddH2OUp to 25

Reaction program

Cycle stepTemp.TimeCycles
Contamination Digestion37 °C5 min1
Initial denaturation95 °C5 min1
Denaturation95 °C15 sec45
Annealing/Extension60 °C30 sec

[Note]:

  1. Amplification Reaction: Adjust the amplification reaction temperature based on the designed primer Tm value.
  2. Fluorescence Signal Acquisition: Different qPCR instruments require different fluorescence signal acquisition times. Please set according to the shortest time limit.

 

Notes

  1. This product is for research use only.
  2. For your safety and health, please wear laboratory attire and disposable gloves while handling.

Reviews

There are no reviews yet.

Be the first to review “Medino® Superpro I TaqMan qPCR Master Mix(UDG plus)”

Your email address will not be published. Required fields are marked *