Medino® Taq DNA Polymerase (100 U/μL)

Product nameMedino® Taq DNA Polymerase (100 U/μL)
Cat.No. LM203025
Size100 kU/1,000 kU/10,000 kU
Storage and shippingThe product is shipped with ice pack and can be stored at -25℃ ~ -15 ℃ for  2 year.

Description

Product description


Medino® Taq DNA Polymerase is a thermostable recombinant DNA polymerase expressed from the thermophilic bacterium Thermus aquaticus, with a molecular weight of 94 kDa. It possesses 5’→3′ polymerase activity and 5’→3′ exonuclease activity but lacks 3’→5′ exonuclease activity. The amplification products have a 3′-dA overhang, making them suitable for direct use in TA cloning.

 

Product Components:

 

Components No.Components
LM203025-AMedino® Taq DNA Polymerase (5 U/μL)

 

Instructions

  1. Recommended PCR Reaction System(Preparation on ice)
Components Volume μLFinal concentration
10 × Medino® PCR Buffer (Mg2+ Free)5
25 mM MgCl231.5 mM
dNTP Mix (10 mM each)10.2 mM
Primer 1 (10 μM)20.4 μM
Primer 2 (10 μM)20.4 μM
Medino® Taq DNA Polymerase (100 U/μL)0.020.04 U/μL
DNA Template100 pg-100 ng
ddH2OTo 50

[Note]:

The amounts of DNA, primers, and probe concentrations provided are recommended starting points. These can be adjusted according to the specific requirements of your experiment to achieve optimal results.

 

2.Reaction program

Cycle stepTemp.TimeCycles
Initial denaturation94 °C30 sec -5 min1
Denaturation94 °C30 sec35
Annealing50 – 60 °C30 sec
Extension72 °C60 sec/kb
Final extension72 °C10 min1

[Note]:

The annealing and extension conditions can be adjusted based on the specific requirements of your experiment to achieve optimal results.

 

Notes

  1. This product is for research use only.
  2. Please operate with lab coats and disposable gloves for your safety.
Documents

Reviews

There are no reviews yet.

Be the first to review “Medino® Taq DNA Polymerase (100 U/μL)”

Your email address will not be published. Required fields are marked *