Medino® Universal TaqMan Multiplex qPCR Master Mix(UDG plus)

ProductMedino® Universal TaqMan Multiplex qPCR Master Mix(UDG plus)
Catalog No.LM601015
Storage conditionsThis product should be stored at -25℃ ~ -15℃ for 1 years.

Description

Product description Medino® Universal TaqMan Multiplex qPCR Master Mix (UDG plus) is a quantitative premix of the Taq enzyme using a new generation antibody method.5 Mix premixed reagent contains reaction buffer and enzyme mix, using dUTP instead of dTTP, and added factors that effectively inhibit non-specific PCR amplification and factors that improve the amplification efficiency of multiplex qPCR reactions, greatly reducing non-specific PCR amplification and contamination, which can ensure primer efficiency while performing up to quadruplex reactions. This product contains the UDG enzyme, which can effectively prevent the risk of aerosol contamination.   Components
Cat.No.LM601015
Size1 mL/5×1 mL
  Instructions
  1. Recommended PCR reaction systems.
ComponentsVolume (μL)Final concentration
5× TaqMan qPCR mix5
Primer mix (10 μM)X0.1 μmol/L – 0.5 μmol/L
Probe mix (10 μM)X50 – 250 nM
ddH2OUp to 25
Template1-10
 
  1. Applicable model
Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne ™ , StepOne Plus ™ , 7500, 7500 Fast, ViiA ™ 7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.  
  1. Reaction program
Cycle stepTemp.TimeCycles
Pollution degradation37℃5 min1
Predenaturation97℃30 sec1
Denaturation97℃3 sec45
Annealing60℃15 sec
Extension60℃15 sec
Notes
  1. a) For your safety and health, please wear a lab coat and disposable gloves during operation.
  2. b) This product is for research use only!
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