RNA Isolation Kit (Purification Column-Based)

Artikel Nr: R6309

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 Important Product Information

  • This kit could isolate high pure RNA within 20
  • The whole experimental process should be carried out on
  • Repeat freezing and thawing for samples may promote RNA degradation, please use fresh samples or samples stored at -80 ℃ to isolate
  • Please use RNase-free appliances and consumables, such as microcentrifuge tubes, tips and
  • The absorbance values at 260 nm, 320 nm, 230 nm and 280 nm represent the absorbance values of nucleic acid, background (solution turbidity), salt concentration and protein and protein, respectively. Therefore, the ratio of OD260/OD280 (R) was usually used to judge RNA purity. The values of R for RNA with high purity are usually within the range of 1.8-2.2, especially 2.0-2.2. When R <1.8, the isolated RNA usually contains protein contamination. However, when R>2.2, few RNA may have been degraded into single
  • RNA quality may also be determined by agarose gel electrophoresis, high-quality total RNA has the following characteristics: clear electrophoresis band, no tailing or blurring; The brightness of 28 s band should be obviously greater than 18 s band, and the ratio is close to or more than 2:1. The brightness of 5 s band is weak or invisible.
  • RNA isolated by this kit contains 28 s and 18 s RNA, but not 5 s RNA, and please use microRNA Isolation Kit (Omn-04) to isolate 5 s RNA, including microRNA and siRNA,
  • gDNA Clear Column can effectively remove genomic DNA, and improve the RNA
  • Please wear lab clothes, gloves and safety glasses during the

Product Introduction

The kit could rapidly isolate RNA from cultured cells and animal tissues based on the unique Lysis Buffer and RNA Purification Column. Moreover, gDNA Clear Column efficiently remove genomic DNA from lysate, which facilitate the isolation of high pure RNA. The isolated RNA could be used for various subsequent experiments, including reverse transcription, real-time fluorescence quantitative PCR (QPCR), Northern Blot, sequencing and other experiments.

Transportation and Storage

This product is transported and stored at room temperature, and valid for 2 years.



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