RNA Isolation Reagent (TRIZOL)

Artikel Nr: R6308

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Important Product Information

  • If samples have a high content of fat, protein and polysaccharide, centrifuge the lysate for 5 minutes at 12,000 g at ℃, then transfer the clear supernatant to a new
  • If the samples are small, please use RNA Purification Mini Kit (Magnetic Beads-Based) (Omn-12) to isolate RNA.
  • The whole experimental process should be carried out on
  • Repeat freezing and thawing for samples may promote RNA degradation, please use fresh samples or samples stored at -80 ℃ to isolate
  • Please use RNase-free appliances and consumables, such as microcentrifuge tubes, tips and
  • The absorbance values at 260 nm, 320 nm, 230 nm and 280 nm represent the absorbance values of nucleic acid, background (solution turbidity), salt concentration and protein and protein, respectively. Therefore, the ratio of OD260/OD280 (R) was usually used to judge RNA purity. The values of R for RNA with high purity are usually within the range of 1.8-2.2, especially 2.0-2.2. When R <1.8, the isolated RNA usually contains protein contamination. However, when R>2.2, few RNA may have been degraded into single
  • RNA quality may also be determined by agarose gel electrophoresis, high-quality total RNA has the following characteristics: clear electrophoresis band, no tailing or blurring; The brightness of 28 s band should be obviously greater than 18 s band, and the ratio is close to or more than 2:1. The brightness of 5 s band is weak or invisible.
  • Please wear lab clothes, gloves and safety glasses to avoid chemical burns of skin, eyes or respiratory tract caused by reagent splash. In case of contact with skin or eyes, please immediately rinse with plenty of water and go to the hospital for

Product Introduction

The Omiget™ RNA Isolation Reagent (TRIZOL) enables isolation of total RNA from various samples, including cell, bacteria, blood, animal and plant tissues. The isolated total RNA could be used for various subsequent experiments, including reverse transcription, real-time fluorescence quantitative PCR (QPCR), Northern Blot, sequencing and other experiments.

Transportation and Storage

This product is transported at room temperature, stored at 4 ℃ and valid for 2 years.

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