EXOBX® Serum/Plasma Exosome Rapid Purification Kit (Magnetic Beads-Based)
Purify exosomes from serum or plasma in approximately 2 hours using modified magnetic beads and a protein-removal step. The handbook includes TEM and NTA characterization of plasma-derived preparations.
~2-hour purification
Protein removal
TEM & NTA data
RUO
Product overview
EXOBX® Serum/Plasma Exosome Rapid Purification Kit (Magnetic Beads-Based) (R6310). The kit captures exosomes from serum or plasma with surface-modified magnetic beads after removal of contaminating proteins using Buffer EXH. Protocols are provided for 0.3, 0.5 and 1 mL sample inputs. Bound vesicles are released with Buffer EXE. The handbook reports typical vesicle morphology by TEM and particle characterization by NTA. A refrigerated centrifuge, magnetic stand and rotator are used in the workflow.
Serum and plasma purification
Proportional reagent systems are provided for 0.3, 0.5 and 1 mL sample volumes.
Protein-removal step
Buffer EXH is intended to reduce albumin, globulin and other contaminants before exosome capture.
Characterization evidence
The handbook includes TEM images and a ZetaView NTA report for an extracted plasma sample.
Specifications
| Parameter | Value |
|---|---|
| Target | Exosomes from serum or plasma |
| Method | Protein depletion, magnetic-bead capture and elution |
| Sample inputs | 0.3, 0.5 or 1 mL |
| Reported processing time | Approximately 2 hours |
| Binding incubation | 90 min at 4°C on a rotator |
| Elution | 1 mL Buffer EXE for the 1 mL sample protocol; scalable |
| Kit buffers | EXA 100 mL; EXE 20 mL; EXH 12 mL |
| Magnetic beads | 5 mL |
| Filters | 10 syringe filters and 10 column-filter sets |
| Storage / shipping | 4°C / ice packs; do not freeze beads |
| Shelf life | 12 months |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Supply | Pack contents |
|---|---|---|
| R6310 | 1 kit | EXA 100 mL; EXE 20 mL; EXH 12 mL; beads 5 mL; 10 syringe filters; 10 column-filter sets |
The handbook does not state a nominal preparation count. Confirm the sales pack before ordering. Source: R6310 handbook, pp. 2–6.
Performance & validation
In the reported plasma application, TEM showed vesicles with typical cup-shaped or concave morphology. The embedded ZetaView NTA report lists a number-based median diameter of 108 nm and an original concentration of 4.5 × 10¹⁰ particles/mL for the analyzed preparation. These sample-specific results are not a capture-rate measurement or a guaranteed yield. Source: R6310 handbook, pp. 2–6.
TEM morphology
The application images show cup-shaped or concave vesicles and multiple particles in a low-magnification field.
NTA characterization
The embedded report gives a 108 nm number-based median diameter and 4.5 × 10¹⁰ particles/mL original concentration.
Approximately 2 hours
The handbook reports an approximately 2-hour purification workflow, including a 90-minute binding incubation.
Product documents
Product FAQs
It purifies exosomes. Use a suitable RNA extraction kit to isolate RNA from the recovered vesicles. Serum and plasma, with protocols for 0.3, 0.5 and 1 mL input. No. Particle concentration and capture/recovery percentage are different measurements; no recovery percentage is supplied. A nominal count is not stated. The handbook lists reagent volumes and 10 sets of each filter type; confirm the sales pack. No. Store at 4°C; freezing can inactivate the beads. No. For scientific research use only, not diagnostic or clinical procedures.Does this kit isolate miRNA?
What samples are supported?
Is the reported particle concentration a recovery rate?
How many preparations are in one kit?
Can I freeze the magnetic beads?
Is this product for diagnostic use?