2×AMPQX® AdvanceFast PCR Master Mix
A 2× high-fidelity PCR premix for rapid amplification and blunt-ended cloning.
High-fidelity amplification
Blunt-ended products
RUO
Product overview
2×AMPQX® AdvanceFast PCR Master Mix. A 2× high-fidelity PCR premix for rapid amplification and blunt-ended cloning.
Fast extension
The reference protocol uses 5–10 seconds per kb at 72°C.
High-fidelity amplification
Designed for accurate amplification of DNA templates.
Blunt-ended products
Suitable for blunt-end cloning; add an A-tail separately when required.
Specifications
| Parameter | Value |
|---|---|
| Concentration | 2× |
| Final Mg²⁺ / dNTPs | 2 mM / 200 µM each |
| Storage | −25 to −15°C, 1 year; dry-ice shipment. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM201001-1 | 1 mL |
| LM201001-1 | 5 × 1 mL |
Source: LM201001-1_2×AMPQX® AdvanceFast PCR Master Mix_10163ES.pdf, pp. 2–3.
Performance & validation
The reference protocol uses 5–10 seconds per kb at 72°C. Designed for accurate amplification of DNA templates. Suitable for blunt-end cloning; add an A-tail separately when required. Source: LM201001-1 handbook/CoA, pp. 2–3.
Fast extension
The reference protocol uses 5–10 seconds per kb at 72°C.
High-fidelity amplification
Designed for accurate amplification of DNA templates.
Blunt-ended products
Suitable for blunt-end cloning; add an A-tail separately when required.
Product documents
Product FAQs
A 2× high-fidelity PCR premix for rapid amplification and blunt-ended cloning. −25 to −15°C, 1 year; dry-ice shipment. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?