qNTQX® qPCR SYBR Green Master Mix (Antibody, High Rox)
A 2× SYBR Green I qPCR premix using antibody-mediated hot-start taq, supplied in the Antibody, High ROX formulation.
Hot-start control
Antibody, High ROX
RUO
Product overview
qNTQX® qPCR SYBR Green Master Mix (Antibody, High Rox). A 2× SYBR Green I qPCR premix using antibody-mediated hot-start taq, supplied in the Antibody, High ROX formulation.
Simple setup
Add target-specific primers, DNA or cDNA template and water.
Hot-start control
The formulation is designed to suppress non-specific amplification and support low-template amplification.
Antibody, High ROX
High-ROX platforms such as StepOne and ABI 7300.
Specifications
| Parameter | Value |
|---|---|
| Concentration | 2× |
| Detection | SYBR Green I |
| Enzyme | Antibody-mediated hot-start Taq |
| Reaction volume | 20 or 50 µL |
| Specificity check | Melt-curve analysis |
| Storage | −20°C, 18 months; protect from light and avoid repeated freeze–thaw. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM601008 | 1 mL |
| LM601008 | 5 × 1 mL |
| LM601008 | 50 × 1 mL |
| LM601008 | 100 × 1 mL |
Source: LM601008_qNTQX_qPCR_SYBR_Green_Master_Mix_Antibody_High_Rox_11200ES.pdf, pp. 2–4.
Performance & validation
Add target-specific primers, DNA or cDNA template and water. The formulation is designed to suppress non-specific amplification and support low-template amplification. High-ROX platforms such as StepOne and ABI 7300. Source: LM601008 handbook/CoA, pp. 2–4.
Simple setup
Add target-specific primers, DNA or cDNA template and water.
Hot-start control
The formulation is designed to suppress non-specific amplification and support low-template amplification.
Antibody, High ROX
High-ROX platforms such as StepOne and ABI 7300.
Product documents
Product FAQs
A 2× SYBR Green I qPCR premix using antibody-mediated hot-start taq, supplied in the Antibody, High ROX formulation. No. Prepare cDNA in a separate reverse-transcription reaction. −20°C, 18 months; protect from light and avoid repeated freeze–thaw. No. It is for research use only.What is this product used for?
Can I use RNA directly?
How should the product be stored?
Is this product intended for diagnostic or clinical use?