qNTQX® Advanced One Step RT-qPCR SYBR Green Kit
A one-step SYBR Green RT-qPCR kit for RNA quantification using gene-specific primers in a single tube.
Dye-based detection
Reported input sensitivity
RUO
Product overview
qNTQX® Advanced One Step RT-qPCR SYBR Green Kit. A one-step SYBR Green RT-qPCR kit for RNA quantification using gene-specific primers in a single tube.
One-tube RNA workflow
Heat-resistant reverse transcriptase and HotStart Taq combine cDNA synthesis and qPCR.
Dye-based detection
SYBR Green I detection uses gene-specific primers and includes melt-curve analysis.
Reported input sensitivity
The handbook reports 0.1 pg for a highly expressed target and 1 pg for a moderately expressed target.
Specifications
| Parameter | Value |
|---|---|
| Detection | SYBR Green I |
| Components | 2× Advanced SG Buffer and Advanced UH Enzyme Mix |
| Reference reverse transcription | 50°C for 6 min |
| Storage | −25 to −15°C, 1 year. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM601011 | 20 T |
| LM601011 | 200 T |
Source: LM601011_qNTQX_Advanced_One_Step_RT-qPCR_SYBR_Green_Kit_11175ES.pdf, pp. 2–4.
Performance & validation
The handbook reports detection at 0.1 pg for a highly expressed target and 1 pg for a moderately expressed target. These are target-dependent claims, not a universal detection limit. Source: LM601011 handbook/CoA, pp. 2–4.
One-tube RNA workflow
Heat-resistant reverse transcriptase and HotStart Taq combine cDNA synthesis and qPCR.
Dye-based detection
SYBR Green I detection uses gene-specific primers and includes melt-curve analysis.
Reported input sensitivity
The handbook reports 0.1 pg for a highly expressed target and 1 pg for a moderately expressed target.
Product documents
Product FAQs
A one-step SYBR Green RT-qPCR kit for RNA quantification using gene-specific primers in a single tube. −25 to −15°C, 1 year. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?