qNTQX® Fast Cell Direct Probe RT-qPCR Kit
A cell-direct probe RT-qPCR kit for gene-expression analysis without RNA extraction, using separate lysis, reverse transcription and qPCR steps.
Reported 1.5-hour workflow
Low cell input
RUO
Product overview
qNTQX® Fast Cell Direct Probe RT-qPCR Kit. A cell-direct probe RT-qPCR kit for gene-expression analysis without RNA extraction, using separate lysis, reverse transcription and qPCR steps.
Cell-direct analysis
Supports cultured animal cells, including primary cells, stem cells and iPS cells.
Reported 1.5-hour workflow
The handbook describes as little as 1.5 hours from template preparation through expression analysis.
Low cell input
The specified 50 µL lysis system accepts 10²–10⁵ cells.
Specifications
| Parameter | Value |
|---|---|
| Detection | Probe-based qPCR |
| Included steps | Cell lysis, DNase treatment, reverse transcription and qPCR |
| Reverse transcription | 37°C for 5 min, then 85°C for 5 s |
| Storage | Unopened A/B and other components: −25 to −15°C. After thawing A/B: 4°C, protected from contamination. Shelf life: 1 year. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM602007 | 40 T |
| LM602007 | 100 T |
Source: LM602007_qNTQX_Fast_Cell_Direct_Probe_RT-qPCR_Kit_11173ES.pdf, pp. 2–4.
Performance & validation
Supports cultured animal cells, including primary cells, stem cells and iPS cells. The handbook describes as little as 1.5 hours from template preparation through expression analysis. The specified 50 µL lysis system accepts 10²–10⁵ cells. Source: LM602007 handbook/CoA, pp. 2–4.
Cell-direct analysis
Supports cultured animal cells, including primary cells, stem cells and iPS cells.
Reported 1.5-hour workflow
The handbook describes as little as 1.5 hours from template preparation through expression analysis.
Low cell input
The specified 50 µL lysis system accepts 10²–10⁵ cells.
Product documents
Product FAQs
What is this product used for?
A cell-direct probe RT-qPCR kit for gene-expression analysis without RNA extraction, using separate lysis, reverse transcription and qPCR steps.
How should the product be stored?
Unopened A/B and other components: −25 to −15°C. After thawing A/B: 4°C, protected from contamination. Shelf life: 1 year.
Is this product intended for diagnostic or clinical use?
No. It is for research use only.