qNTQX® qPCR SYBR Green Master Mix (High Rox Plus)
A 2× SYBR Green I qPCR premix using ligand-regulated hot-start polymerase, supplied in the High ROX Plus formulation.
Hot-start control
High ROX Plus
RUO
Product overview
qNTQX® qPCR SYBR Green Master Mix (High Rox Plus). A 2× SYBR Green I qPCR premix using ligand-regulated hot-start polymerase, supplied in the High ROX Plus formulation.
Simple setup
Add target-specific primers, DNA or cDNA template and water.
Hot-start control
The formulation is designed to suppress non-specific amplification and support low-template amplification.
High ROX Plus
High-ROX platforms such as StepOne and ABI 7300.
Specifications
| Parameter | Value |
|---|---|
| Concentration | 2× |
| Detection | SYBR Green I |
| Enzyme | Ligand-regulated hot-start polymerase |
| Reaction volume | 20 or 50 µL |
| Specificity check | Melt-curve analysis |
| Storage | −25 to −15°C, 18 months; protect from light and avoid repeated freeze–thaw. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM601005 | 1 mL |
| LM601005 | 5 × 1 mL |
| LM601005 | 50 × 1 mL |
| LM601005 | 100 × 1 mL |
Source: LM601005_qNTQX_qPCR_SYBR_Green_Master_Mix_High_Rox_Plus_11203ES.pdf, pp. 2–4.
Performance & validation
Add target-specific primers, DNA or cDNA template and water. The formulation is designed to suppress non-specific amplification and support low-template amplification. High-ROX platforms such as StepOne and ABI 7300. Source: LM601005 handbook/CoA, pp. 2–4.
Simple setup
Add target-specific primers, DNA or cDNA template and water.
Hot-start control
The formulation is designed to suppress non-specific amplification and support low-template amplification.
High ROX Plus
High-ROX platforms such as StepOne and ABI 7300.
Product documents
Product FAQs
A 2× SYBR Green I qPCR premix using ligand-regulated hot-start polymerase, supplied in the High ROX Plus formulation. No. Prepare cDNA in a separate reverse-transcription reaction. −25 to −15°C, 18 months; protect from light and avoid repeated freeze–thaw. No. It is for research use only.What is this product used for?
Can I use RNA directly?
How should the product be stored?
Is this product intended for diagnostic or clinical use?