NUCQX® Magnetic Bead-Based Tissue Genomic DNA Extraction Kit
Extract genomic DNA from fresh or frozen animal tissues using Proteinase K lysis and magnetic-bead purification. Pre-aliquoted and bulk formats support automated or manual processing.
Fresh or frozen samples
30–50 mg tissue input
Proteinase K included
RUO
Product overview
LM102112 NUCQX® Magnetic Bead-Based Tissue Genomic DNA Extraction Kit is designed for fresh or frozen animal muscle, internal organs and other tissues. The protocol combines tissue disruption, Proteinase K digestion, magnetic-bead binding, washing and elution. It provides automated and manual extraction procedures and an optional RNase A treatment. Purified extracts can be used for downstream experiments. The workflow avoids phenol and chloroform.
Fresh or frozen tissue
The handbook specifies animal muscle, internal organs and other tissues, with 30–50 mg fresh-weight input in the protocol.
Proteinase K lysis
Proteinase K is included. Tissue lysis is performed at 65°C for 45 minutes before purification.
Optional RNA removal
An optional RNase A step supports workflows requiring RNA removal. RNase A and isopropanol are user-supplied.
Specifications
| Parameter | Value |
|---|---|
| Target | Animal tissue genomic DNA |
| Sample types | Fresh or frozen muscle, internal organs and other tissues |
| Tissue input | 30–50 mg fresh weight |
| Lysis | 400 µL Lysis Buffer + 10 µL Proteinase K; 65°C, 45 min |
| Cleared lysate loaded | 300 µL |
| Binding additions | 300 µL Binding Buffer + 400 µL isopropanol |
| Elution volume | 100 µL |
| User-supplied reagents | Isopropanol; RNase A (optional RNA removal) |
| Storage | 10–30°C for all kit components |
| Shelf life | 12 months |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Preparations | Supply format |
|---|---|---|
| LM102112ES01 | 16 | Pre-aliquoted |
| LM102112ES02 | 96 | Pre-aliquoted |
| LM102112ES03 | 20 | Bulk |
| LM102112ES04 | 100 | Bulk |
| LM102112ES05 | 500 | Bulk |
State the required pack size, format and extractor model when ordering. Source: LM102112 NUCQX® Magnetic Bead-Based Tissue Genomic DNA Extraction Kit handbook, pp. 2–6.
Performance & validation
Designed to extract genomic DNA from fresh or frozen animal muscle, internal organs and other tissues. Proteinase K lysis releases nucleic acids for magnetic-bead capture, washing and elution. The handbook describes the eluate as suitable for downstream experiments, with results dependent on the sample and procedure. Source: LM102112 handbook, pp. 2–6.
Animal tissue genomic DNA
The protocol processes 30–50 mg fresh-weight tissue. This is the recommended input amount, not the recovered DNA mass.
Lysis and impurity removal
Proteinase K digestion is followed by magnetic-bead purification. Optional RNase A treatment is provided for RNA removal.
100 µL eluate
Both workflows use 100 µL Elution Buffer. The handbook does not report a numerical DNA capture or recovery rate.
Product documents
Product FAQs
The pack table lists S-16 with 16 T, S-96 with 96 T and S-48 with 100 T bulk. N-96 programmes are provided but its pack mapping is unclear; confirm before ordering. Use 30–50 mg fresh-weight animal tissue and follow the disruption and lysis procedure. No. It is the tissue lysis incubation. Sample disruption, purification and elution require additional time. Proteinase K is included. RNase A for optional RNA removal and isopropanol must be supplied separately. Store the kit at 10–30°C for up to 12 months. The handbook specifies −20°C for longer-term eluate storage. No. It is for scientific research use only, not diagnostic or clinical procedures.Which pack should I order for my instrument?
How much tissue is recommended?
Is 45 minutes the total extraction time?
Are Proteinase K and RNase A included?
How should I store the kit and eluate?
Is the kit intended for diagnostic use?