2×qNTQX® Universal Advanced qPCR SYBR Master Mix (UDG plus)
A 2× SYBR Green qPCR premix with chemically modified hot-start polymerase, dUTP/UDG and a special ROX reference dye.
Carryover control
Universal ROX formulation
RUO
Product overview
qNTQX® Universal Advanced qPCR SYBR Master Mix (UDG plus). A 2× SYBR Green qPCR premix with chemically modified hot-start polymerase, dUTP/UDG and a special ROX reference dye.
Chemically modified hot start
Designed to suppress non-specific products and primer dimers.
Carryover control
dUTP and UDG help limit carryover from previously amplified products.
Universal ROX formulation
The handbook describes a special ROX dye intended to avoid instrument-specific concentration adjustment.
Specifications
| Parameter | Value |
|---|---|
| Reaction volume | 20 µL reference system |
| Mix dose | 10 µL per 20 µL reaction |
| Readout | SYBR Green I with melt curve |
| Storage | −25 to −15°C, 24 months; protect from light. Ship with ice packs. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM601017 | 5 mL |
| LM601017 | 5 × 5 mL |
The 5 mL pack is listed as 4 × 1.25 mL (500 reactions at 20 µL); the 25 mL pack contains five such sets (2,500 reactions). Source: LM601017_qNTQX_Universal_Advanced_qPCR_SYBR_Master_Mix_UDG_plus_A411.pdf, pp. 2–4.
Performance & validation
Designed to suppress non-specific products and primer dimers. dUTP and UDG help limit carryover from previously amplified products. The handbook describes a special ROX dye intended to avoid instrument-specific concentration adjustment. Source: LM601017 handbook/CoA, pp. 2–4.
Chemically modified hot start
Designed to suppress non-specific products and primer dimers.
Carryover control
dUTP and UDG help limit carryover from previously amplified products.
Universal ROX formulation
The handbook describes a special ROX dye intended to avoid instrument-specific concentration adjustment.
Product documents
Product FAQs
A 2× SYBR Green qPCR premix with chemically modified hot-start polymerase, dUTP/UDG and a special ROX reference dye. −25 to −15°C, 24 months; protect from light. Ship with ice packs. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?