mRNex® E.coli poly(A) Polymerase (5 U/μL)
E. coli poly(A) polymerase for template-independent addition of poly(A) tails to single-stranded RNA.
Template-independent reaction
Buffer included
RUO
Product overview
mRNex® E.coli poly(A) Polymerase (5 U/μL). E. coli poly(A) polymerase for template-independent addition of poly(A) tails to single-stranded RNA.
RNA 3′ tailing
Adds AMP residues to the 3′ end of RNA.
Template-independent reaction
Uses ATP without requiring a DNA template.
Buffer included
Supplied with 10× reaction buffer.
Specifications
| Parameter | Value |
|---|---|
| Activity concentration | 5 U/µL |
| Substrate | Single-stranded RNA |
| Reference temperature | 37°C |
| Storage | −25 to −15°C, 2 years. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LD303001 | 100 U |
| LD303001 | 500 U |
Source: LD303001_mRNex® E.coli poly(A) Polymerase (5 U-μL)_14801ES.pdf, pp. 2–3.
Performance & validation
Adds AMP residues to the 3′ end of RNA. Uses ATP without requiring a DNA template. Supplied with 10× reaction buffer. Source: LD303001 handbook/CoA, pp. 2–3.
RNA 3′ tailing
Adds AMP residues to the 3′ end of RNA.
Template-independent reaction
Uses ATP without requiring a DNA template.
Buffer included
Supplied with 10× reaction buffer.
Product documents
Product FAQs
E. coli poly(A) polymerase for template-independent addition of poly(A) tails to single-stranded RNA. DNA is not a substrate. The handbook does not recommend double-stranded RNA or very short oligonucleotides. −25 to −15°C, 2 years. No. It is for research use only.What is this product used for?
Can DNA or double-stranded RNA be used?
How should the product be stored?
Is this product intended for diagnostic or clinical use?