mRNex® Gold T4 DNA Ligase (5 U/µL)
T4 DNA ligase for ligating compatible nucleic-acid ends in cloning and related molecular-biology workflows.
Multiple applications
Buffer included
RUO
Product overview
mRNex® Gold T4 DNA Ligase (5 U/µL). T4 DNA ligase for ligating compatible nucleic-acid ends in cloning and related molecular-biology workflows.
Blunt and sticky ends
Catalyses phosphodiester-bond formation in double-stranded DNA.
Multiple applications
Supports DNA oligonucleotide ligation, cDNA cloning and compatible RNA labelling/circularization workflows.
Buffer included
Supplied with 10× ligase buffer.
Specifications
| Parameter | Value |
|---|---|
| Activity concentration | 5 U/µL |
| Required end chemistry | 5′ phosphate and 3′ hydroxyl |
| Cofactor | ATP |
| Storage | −20°C, 2 years. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LD304001 | 1,000 U |
| LD304001 | 50,000 U |
Source: LD304001_mRNex® Gold T4 DNA Ligase (5 U-µL)_10300ES.pdf, pp. 2–3.
Performance & validation
Catalyses phosphodiester-bond formation in double-stranded DNA. Supports DNA oligonucleotide ligation, cDNA cloning and compatible RNA labelling/circularization workflows. Supplied with 10× ligase buffer. Source: LD304001 handbook/CoA, pp. 2–3.
Blunt and sticky ends
Catalyses phosphodiester-bond formation in double-stranded DNA.
Multiple applications
Supports DNA oligonucleotide ligation, cDNA cloning and compatible RNA labelling/circularization workflows.
Buffer included
Supplied with 10× ligase buffer.
Product documents
Product FAQs
T4 DNA ligase for ligating compatible nucleic-acid ends in cloning and related molecular-biology workflows. The handbook states that ligation requires a compatible double-stranded region; fully single-stranded sequences are not ligated. −20°C, 2 years. No. It is for research use only.What is this product used for?
Does it ligate fully single-stranded sequences?
How should the product be stored?
Is this product intended for diagnostic or clinical use?