mRNex® Plus One Step Cloning Kit
A homology-based one-step kit for directional cloning of PCR fragments into linearized vectors.
Directional assembly
Controls included
RUO
Product overview
mRNex® Plus One Step Cloning Kit. A homology-based one-step kit for directional cloning of PCR fragments into linearized vectors.
Wide insert range
The handbook describes inserts from 50 bp to 10 kb.
Directional assembly
Uses 15–25 bp end homology with a linearized vector.
Controls included
Supplied with a control insert and linearized pUC19 control vector.
Specifications
| Parameter | Value |
|---|---|
| Premix | 2× Fast Clone Enzyme Premix |
| Applications | Directional cloning and site-directed mutagenesis |
| Storage | −20°C, 1 year; dry-ice shipment. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM301002 | 5 T |
| LM301002 | 20 T |
| LM301002 | 50 T |
Source: LM301002_mRNex® Plus One Step Cloning Kit_10911ES.pdf, pp. 2–9.
Performance & validation
The handbook reports cloning efficiency above 95% under its conditions. Efficiency depends on fragment design, DNA quality and transformation performance. Source: LM301002 handbook/CoA, pp. 2–9.
Wide insert range
The handbook describes inserts from 50 bp to 10 kb.
Directional assembly
Uses 15–25 bp end homology with a linearized vector.
Controls included
Supplied with a control insert and linearized pUC19 control vector.
Product documents
Product FAQs
A homology-based one-step kit for directional cloning of PCR fragments into linearized vectors. −20°C, 1 year; dry-ice shipment. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?