mRNex® Precise restriction enzyme XbaI
Recombinant XbaI restriction enzyme for sequence-specific cleavage of plasmid DNA, PCR products and genomic DNA.
Shared buffer system
Template-specific timing
RUO
Product overview
mRNex® Precise restriction enzyme XbaI. Recombinant XbaI restriction enzyme for sequence-specific cleavage of plasmid DNA, PCR products and genomic DNA.
Sequence-specific cleavage
Recognises the TCTAGA site.
Shared buffer system
Includes SharpSeq Buffer and a colour-buffer option for direct gel loading.
Template-specific timing
The protocol specifies 15 min for plasmid, 15–30 min for PCR products and 30–60 min for genomic DNA.
Specifications
| Parameter | Value |
|---|---|
| Activity concentration | 20 U/µL |
| Reaction temperature | 37°C |
| Heat inactivation | 80°C for 20 min |
| Storage | −20°C, 2 years; dry-ice shipment. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LD101006 | 500 T |
Source: LD101006_mRNex® Precise restriction enzyme XbaI_15033ES.pdf, pp. 2–4.
Performance & validation
Recognises the TCTAGA site. Includes SharpSeq Buffer and a colour-buffer option for direct gel loading. The protocol specifies 15 min for plasmid, 15–30 min for PCR products and 30–60 min for genomic DNA. Source: LD101006 handbook/CoA, pp. 2–4.
Sequence-specific cleavage
Recognises the TCTAGA site.
Shared buffer system
Includes SharpSeq Buffer and a colour-buffer option for direct gel loading.
Template-specific timing
The protocol specifies 15 min for plasmid, 15–30 min for PCR products and 30–60 min for genomic DNA.
Product documents
Product FAQs
Recombinant XbaI restriction enzyme for sequence-specific cleavage of plasmid DNA, PCR products and genomic DNA. Yes. Overlapping Dam methylation can block cleavage, as noted in the handbook. −20°C, 2 years; dry-ice shipment. No. It is for research use only.What is this product used for?
Can methylation affect digestion?
How should the product be stored?
Is this product intended for diagnostic or clinical use?