ISOQX® LAMP Fluorescent Assay Kit (Bst Plus HSS)
Hot-start LAMP amplification with aptamer-blocked Bst Plus HSS polymerase. Separately supplied SYTO-9 dye supports fluorescent detection and flexibility for probe-based assay development.
Fluorescent LAMP
Separate SYTO-9 dye
Room-temperature setup
RUO
Product overview
ISOQX® LAMP Fluorescent Assay Kit (Bst Plus HSS) (LM701102). This kit is designed for DNA-template isothermal amplification with Bst Plus HSS DNA Polymerase and an optimized buffer. Aptamer-mediated hot start permits reaction setup at room temperature. The fluorescent dye is supplied separately and may be omitted for probe-based detection. The supplied protocol uses 63–65°C incubation with fluorescence acquisition through the SYBR channel.
Room-temperature setup
Aptamer-blocked hot-start Bst Plus HSS polymerase supports convenient reagent preparation.
Separate fluorescent dye
SYTO-9 is provided in its own vial and can be omitted when an assay-specific probe is used.
Isothermal fluorescence monitoring
Collect fluorescence during 63–65°C incubation using the indicated instrument settings.
Specifications
| Parameter | Value |
|---|---|
| Template | DNA in the supplied protocol |
| Enzyme | Bst Plus HSS DNA Polymerase; aptamer-blocked hot start |
| Components | 4× LAMP Buffer, polymerase and 25× fluorescent dye |
| Dye | SYTO-9; supplied separately |
| Reaction volume | 25 µL |
| Incubation | 63–65°C; 30–60 one-minute acquisition intervals |
| Inactivation | 95°C for 3 min |
| Detection channel | SYBR channel in SYBR Green Reagents mode |
| Storage | −25 to −15°C; protect dye from light |
| Shelf life | 1 year |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Tests | Buffer / enzyme / dye |
|---|---|---|
| LM701102ES01 | 100 | 600 µL / 100 µL / 100 µL |
| LM701102ES02 | 1,000 | 6 mL / 1 mL / 1 mL |
State the required pack size when ordering. Source: LM701102 handbook, pp. 2–3.
Performance & validation
The kit supports hot-start fluorescent LAMP with DNA templates. The handbook describes room-temperature setup, separate dye or probe-based detection options, and amplification at 63–65°C. No numerical sensitivity, specificity or time-to-positive validation results are supplied. Source: LM701102 handbook, pp. 2–3.
Hot-start control
Aptamer-blocked polymerase is designed to improve specificity during isothermal assay setup.
Dye or probe detection
Use the supplied SYTO-9 dye, or omit it for an appropriately designed fluorescent-probe assay.
30–60 minute incubation
The reference protocol uses 30–60 one-minute acquisition intervals at 63–65°C, followed by inactivation.
Product documents
Product FAQs
SYTO-9, supplied separately as a 25× stock. Yes. Omit the dye component and validate the probe-based assay. No separate reverse transcriptase is listed. The supplied setup is DNA-based; confirm RT-LAMP requirements before using RNA. The handbook specifies SYBR Green Reagents mode and the SYBR channel. The handbook states 6 µL of 4× buffer in 25 µL as 1×, which calculates to 0.96×. Confirm the intended dose before use. No. For scientific research use only, not diagnostic or clinical procedures.What dye is included?
Can I use a fluorescent probe?
Does the kit include reverse transcriptase?
What fluorescence channel should I select?
Is the buffer dose internally consistent?
Is this product for diagnostic use?