2×AMPQX® Ultra-Rapid HotStart PCR Master Mix (with Dye)
A 2× hot-start PCR premix with dye for ultrafast endpoint amplification.
Long-fragment capability
Direct gel and TA cloning
RUO
Product overview
2×AMPQX® Ultra-Rapid HotStart PCR Master Mix (with Dye). A 2× hot-start PCR premix with dye for ultrafast endpoint amplification.
Ultrafast extension
The handbook reports 1–3 s/kb for complex templates up to 3 kb and 1 s/kb for plasmids up to 5 kb.
Long-fragment capability
Amplification up to 15 kb is described; longer targets require adjusted extension.
Direct gel and TA cloning
Dye is included and products have 3′ A overhangs.
Specifications
| Parameter | Value |
|---|---|
| Concentration | 2× |
| Reference extension | 1–10 s/kb depending on template |
| Storage | −25 to −15°C, 2 years; dry-ice shipment. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM201006-2 | 1 mL |
| LM201006-2 | 5 × 1 mL |
| LM201006-2 | 50 × 1 mL |
| LM201006-2 | 100 × 1 mL |
Source: LM201006-2_2×AMPQX® Ultra-Rapid HotStart PCR Master Mix (with Dye)_10157ES.pdf, pp. 2–3.
Performance & validation
The handbook reports 1–3 s/kb for complex templates up to 3 kb and 1 s/kb for plasmids up to 5 kb. Amplification up to 15 kb is described; longer targets require adjusted extension. Dye is included and products have 3′ A overhangs. Source: LM201006-2 handbook/CoA, pp. 2–3.
Ultrafast extension
The handbook reports 1–3 s/kb for complex templates up to 3 kb and 1 s/kb for plasmids up to 5 kb.
Long-fragment capability
Amplification up to 15 kb is described; longer targets require adjusted extension.
Direct gel and TA cloning
Dye is included and products have 3′ A overhangs.
Product documents
Product FAQs
A 2× hot-start PCR premix with dye for ultrafast endpoint amplification. −25 to −15°C, 2 years; dry-ice shipment. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?