EXOBX® Dual Luciferase Reporter Gene Assay Kit (Enhanced)
An enhanced assay kit for sequential measurement of firefly and Renilla luciferase in the same sample.
Reported analytical sensitivity
Wide reported range
RUO
Product overview
EXOBX® Dual Luciferase Reporter Gene Assay Kit (Enhanced). An enhanced assay kit for sequential measurement of firefly and Renilla luciferase in the same sample.
Dual-reporter readout
Measures firefly followed by Renilla luciferase in one sample.
Reported analytical sensitivity
The handbook reports detection down to 10⁻¹⁹ mol luciferase.
Wide reported range
A linear range of at least seven orders of magnitude is described.
Specifications
| Parameter | Value |
|---|---|
| Readout | Luminescence |
| SFAR function | Stops firefly signal and initiates Renilla measurement |
| Storage | Lysis/SFAR buffers: −20°C; firefly reagent/Renilla substrate: −80°C. Shelf life: 2 years; dry-ice shipment. |
| Intended use | Research use only |
Pack sizes & ordering
| Component | Quantity |
|---|---|
| 5× cell lysis buffer | 20 mL |
| Firefly detection reagent | 10 mL |
| Renilla substrate (50×) | 0.2 mL |
| SFAR buffer | 15 mL |
Quantities are kit contents from the handbook, not separately orderable pack sizes. Test count is not specified. Source: R4101_EXOBX® Dual Luciferase Reporter Gene Assay Kit (Enhanced)_Omt-06.pdf, pp. 2–5.
Performance & validation
The handbook reports sensitivity down to 10⁻¹⁹ mol and a linear range of at least seven orders of magnitude. These are handbook-stated analytical capabilities, not a guaranteed limit for every sample or instrument. Source: R4101 handbook/CoA, pp. 2–5.
Dual-reporter readout
Measures firefly followed by Renilla luciferase in one sample.
Reported analytical sensitivity
The handbook reports detection down to 10⁻¹⁹ mol luciferase.
Wide reported range
A linear range of at least seven orders of magnitude is described.
Product documents
Product FAQs
An enhanced assay kit for sequential measurement of firefly and Renilla luciferase in the same sample. Lysis/SFAR buffers: −20°C; firefly reagent/Renilla substrate: −80°C. Shelf life: 2 years; dry-ice shipment. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?