EXOBX® Magnetic EV/Tissue/Plasma miRNA Kit
Isolate miRNA from tissue, serum/plasma and exosome samples with magnetic-bead purification. Manual and automated workflows support downstream assays and NGS analysis.
Tissue, plasma & exosomes
Manual or automated
NGS-compatible extracts
RUO
Product overview
EXOBX® Magnetic EV/Tissue/Plasma miRNA Kit (R1309). Specially encapsulated magnetic beads and a dedicated buffer system bind miRNA under controlled conditions, remove proteins and other impurities by washing, and release the purified RNA into RNase-free water. The kit supports tissue, serum/plasma and exosome samples. Manual procedures and automated-platform guidance are supplied, including an example for TGuide S16.
Multiple sample types
Protocols cover tissue, serum/plasma and exosome preparations within one kit.
Manual or automated processing
Compatible platforms listed include Kingfisher Flex96, TGuide S32 Pro and TGuide S16.
Downstream RNA applications
The handbook describes purified miRNA as suitable for downstream assays and NGS analysis.
Specifications
| Parameter | Value |
|---|---|
| Target | miRNA |
| Sample types | Tissue, serum/plasma and exosome samples |
| Tissue input | 15 mg |
| Liquid sample input | 100–200 µL; 800 µL scale-up example provided |
| Elution volume | 50–100 µL RNase-free ddH₂O; minimum 50 µL |
| Elution conditions | 56°C for 5 min in the manual protocol |
| Platforms | Kingfisher Flex96; TGuide S32 Pro; TGuide S16; manual magnetic rack |
| User-supplied reagent | Absolute ethanol; add to RDH and RWA per bottle labels |
| Storage | 15–30°C, dry conditions |
| Shelf life | 15 months |
| Purified miRNA storage | −80°C |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Preparations | Format |
|---|---|---|
| R1309ES01 | 24 | Magnetic-bead miRNA purification kit |
| R1309ES02 | 96 | Magnetic-bead miRNA purification kit |
State the required pack size when ordering. Source: R1309 handbook, pp. 2–5.
Performance & validation
The handbook describes magnetic-bead isolation of miRNA from tissue, serum/plasma and exosome samples, with purification intended to reduce protein and other impurities. It supports manual processing and compatible automated platforms, and lists downstream assays and NGS use. Numerical miRNA recovery, yield and purity datasets are not provided. Source: R1309 handbook, pp. 2–5.
miRNA capture
Dedicated buffers enable nucleic acid binding to the magnetic beads and release during elution.
Defined sample inputs
The manual protocol uses 15 mg tissue or 100–200 µL liquid sample, with an 800 µL scale-up example.
50–100 µL eluate
Purified miRNA is eluted in RNase-free water for downstream work. Elution volume is not a recovery-rate measurement.
Product documents
Product FAQs
No. It isolates miRNA from tissue, serum/plasma or exosome samples. A manual magnetic stand or a compatible extractor such as Kingfisher Flex96, TGuide S32 Pro or TGuide S16. Yes. Add absolute ethanol to RDH and RWA according to the bottle labels; ethanol is user supplied. 50–100 µL RNase-free water; the manual specifies a minimum of 50 µL. Store purified miRNA at −80°C. The loading table and later wash/elution positions differ. Confirm the corrected instrument programme before automated use. No. For scientific research use only, not diagnostic or clinical procedures.Does this kit purify intact exosomes?
Which equipment can be used?
Do I add ethanol before use?
What elution volume should I use?
Are automated plate positions consistent?
Is this product for diagnostic use?