Catalog No. LD301007

mRNex® Vaccinia Capping System

An enzyme-based post-transcriptional RNA capping system for generating Cap1-modified mRNA.

Cap1 formation
Complete capping reagents
Cap0 option
RUO

Product overview

mRNex® Vaccinia Capping System. An enzyme-based post-transcriptional RNA capping system for generating Cap1-modified mRNA.

Cap1 formation

Combines vaccinia capping enzyme and 2′-O-methyltransferase.

Complete capping reagents

Includes buffer, GTP, SAM and RNase inhibitor.

Cap0 option

Omit 2′-O-methyltransferase when Cap0 RNA is required.

Specifications

ParameterValue
ApplicationPost-transcriptional RNA capping
Reference RNA input10 µg
Reported reaction durationGenerally within 30 min; RNA-dependent
Storage−25 to −15°C, 1 year.
Intended useResearch use only

Pack sizes & ordering

Catalog No.Pack size
LD30100750 T
LD301007100 T
LD301007500 T

Source: LD301007_mRNex® Vaccinia Capping System_17121ES.pdf, pp. 2–4.

Performance & validation

Combines vaccinia capping enzyme and 2′-O-methyltransferase. Includes buffer, GTP, SAM and RNase inhibitor. Omit 2′-O-methyltransferase when Cap0 RNA is required. Source: LD301007 handbook/CoA, pp. 2–4.

Cap1 formation

Combines vaccinia capping enzyme and 2′-O-methyltransferase.

Complete capping reagents

Includes buffer, GTP, SAM and RNase inhibitor.

Cap0 option

Omit 2′-O-methyltransferase when Cap0 RNA is required.

Product documents

Product FAQs

What is this product used for?

An enzyme-based post-transcriptional RNA capping system for generating Cap1-modified mRNA.

Is capping efficiency the same for every RNA?

No. The handbook states that the RNA 5′ sequence, secondary structure, length and reaction volume affect the reaction.

How should the product be stored?

−25 to −15°C, 1 year.

Is this product intended for diagnostic or clinical use?

No. It is for research use only.

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