qNTQX® Fast Cell Direct SYBR Green RT-qPCR Kit
A cell-direct gene-expression kit combining cell lysis, separate reverse transcription and SYBR Green qPCR without further RNA purification.
Reported 1.5-hour workflow
Defined cell input
RUO
Product overview
qNTQX® Fast Cell Direct SYBR Green RT-qPCR Kit. A cell-direct gene-expression kit combining cell lysis, separate reverse transcription and SYBR Green qPCR without further RNA purification.
Cell-direct workflow
For adherent and suspension animal cells, primary cells, stem cells and iPS cells.
Reported 1.5-hour workflow
The handbook describes as little as 1.5 hours from template preparation through gene-expression analysis.
Defined cell input
The handbook lists validation with 10³–10⁵ cells per 50 µL lysis system.
Specifications
| Parameter | Value |
|---|---|
| Detection | SYBR Green qPCR |
| Workflow | Cell lysis, reverse transcription, then qPCR |
| Storage | −25 to −15°C, 6 months. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM601013 | 40 T |
| LM601013 | 100 T |
Source: LM601013_qNTQX_Fast_Cell_Direct_SYBR_Green_RT-qPCR_Kit_11172ES.pdf, pp. 2–3.
Performance & validation
For adherent and suspension animal cells, primary cells, stem cells and iPS cells. The handbook describes as little as 1.5 hours from template preparation through gene-expression analysis. The handbook lists validation with 10³–10⁵ cells per 50 µL lysis system. Source: LM601013 handbook/CoA, pp. 2–3.
Cell-direct workflow
For adherent and suspension animal cells, primary cells, stem cells and iPS cells.
Reported 1.5-hour workflow
The handbook describes as little as 1.5 hours from template preparation through gene-expression analysis.
Defined cell input
The handbook lists validation with 10³–10⁵ cells per 50 µL lysis system.
Product documents
Product FAQs
A cell-direct gene-expression kit combining cell lysis, separate reverse transcription and SYBR Green qPCR without further RNA purification. −25 to −15°C, 6 months. No. It is for research use only.What is this product used for?
How should the product be stored?
Is this product intended for diagnostic or clinical use?