qNTQX® qPCR SYBR Green Master Mix (Antibody, No Rox)
A 2× SYBR Green I qPCR premix using antibody-mediated hot-start taq, supplied in the Antibody, No ROX formulation.
Hot-start control
Antibody, No ROX
RUO
Product overview
qNTQX® qPCR SYBR Green Master Mix (Antibody, No Rox). A 2× SYBR Green I qPCR premix using antibody-mediated hot-start taq, supplied in the Antibody, No ROX formulation.
Simple setup
Add target-specific primers, DNA or cDNA template and water.
Hot-start control
The formulation is designed to suppress non-specific amplification and support low-template amplification.
Antibody, No ROX
Platforms not requiring ROX normalization, such as Bio-Rad CFX96 and Rotor-Gene Q.
Specifications
| Parameter | Value |
|---|---|
| Concentration | 2× |
| Detection | SYBR Green I |
| Enzyme | Antibody-mediated hot-start Taq |
| Reaction volume | 20 or 50 µL |
| Specificity check | Melt-curve analysis |
| Storage | −25 to −15°C, 18 months; protect from light and avoid repeated freeze–thaw. |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Pack size |
|---|---|
| LM601006 | 1 mL |
| LM601006 | 5 × 1 mL |
| LM601006 | 5 × 5 mL |
| LM601006 | 10 × 5 mL |
| LM601006 | 20 × 5 mL |
Source: LM601006_qNTQX_qPCR_SYBR_Green_Master_Mix_Antibody_No_Rox_11198ES.pdf, pp. 2–4.
Performance & validation
Add target-specific primers, DNA or cDNA template and water. The formulation is designed to suppress non-specific amplification and support low-template amplification. Platforms not requiring ROX normalization, such as Bio-Rad CFX96 and Rotor-Gene Q. Source: LM601006 handbook/CoA, pp. 2–4.
Simple setup
Add target-specific primers, DNA or cDNA template and water.
Hot-start control
The formulation is designed to suppress non-specific amplification and support low-template amplification.
Antibody, No ROX
Platforms not requiring ROX normalization, such as Bio-Rad CFX96 and Rotor-Gene Q.
Product documents
Product FAQs
A 2× SYBR Green I qPCR premix using antibody-mediated hot-start taq, supplied in the Antibody, No ROX formulation. No. Prepare cDNA in a separate reverse-transcription reaction. −25 to −15°C, 18 months; protect from light and avoid repeated freeze–thaw. No. It is for research use only.What is this product used for?
Can I use RNA directly?
How should the product be stored?
Is this product intended for diagnostic or clinical use?