qNTQX® Robust Multiplex qPCR PreMix (UDG Plus)
A 2× probe-based multiplex qPCR premix with antibody-mediated hot-start Taq, inhibitor tolerance and dUTP/UDG carryover control.
Inhibitor tolerance
Fast cycling
RUO
Product overview
qNTQX® Robust Multiplex qPCR PreMix (UDG Plus), catalog no. LM602015, is a probe-based multiplex real-time PCR premix built around antibody-mediated hot-start Taq DNA polymerase. It is designed for broad primer/probe compatibility and tolerance to common PCR inhibitors, including nucleic-acid release reagents. Add assay-specific primers, probes, template and water. A reverse-transcription step is not included.
Robust multiplex chemistry
Designed for probe-based multiplex assays and tolerance to interfering substances such as nucleic-acid release reagents.
Flexible assay preparation
Supports primer/probe pre-mixing and standard or fast cycling. Validate the specific assay and instrument settings.
dUTP/UDG carryover control
Helps control uracil-containing carryover products. Good laboratory practices and negative controls remain necessary.
Specifications
| Parameter | Value |
|---|---|
| Format | 2× TaqMan qPCR Mix |
| Enzyme | Antibody-mediated hot-start Taq DNA polymerase |
| Detection | Probe-based multiplex qPCR |
| Carryover control | dUTP/UDG |
| Reaction volume | 25 µL example; 12.5 µL of 2× mix |
| Template volume | 1–10 µL per 25 µL reaction |
| Final primer concentration | 0.1–0.5 µM |
| Final probe concentration | 0.05–0.25 µM |
| Storage and shelf life | −25 to −15°C; 18 months from manufacture |
| Intended use | Research use only |
Pack sizes & ordering
| Catalog No. | Nominal tests | 2× mix volume |
|---|---|---|
| LM602015 | 100 | 1.25 mL |
| LM602015 | 1,000 | 12.5 mL |
| LM602015 | 10,000 | 125 mL |
| LM602015 | 20,000 | 250 mL |
Test counts correspond to 25 µL reactions using 12.5 µL of 2× mix. Source: LM602015 handbook, pp. 2–3.
Performance & validation
The handbook reports inhibitor tolerance, primer/probe pre-mixing support and detection within ≤35 minutes using a fast programme. No quantitative inhibitor limits or comparative performance plots are supplied. Timing and performance require assay and instrument validation. Source: LM602015 handbook, pp. 2–3.
Fast programme
95°C for 90 s, then 42 cycles of 95°C for 5 s and 60°C for 15 s; handbook programme labelled Q5.
Inhibitor tolerance
Designed to tolerate interfering substances such as nucleic-acid release reagents.
Assay preparation
Primer/probe pre-mixing is supported; storage duration of prepared assay mixes is not specified.
Product documents
Product FAQs
Can the premix amplify RNA directly?
No reverse transcriptase is listed. Use DNA/cDNA or a suitable reverse-transcription workflow.
Is UDG incubation required?
The standard protocol lists an optional 37°C, 5 min step. Follow the handbook and validate your assay.
Is ≤35 min guaranteed on every instrument?
No. The handbook describes a fast programme; confirm timing and performance on your instrument.
How should the product be stored?
Store at −25 to −15°C. Shelf life is 18 months from manufacture.
Is this product for diagnostic use?
No. It is for research use only.