qNTQX® Universal TaqMan Pro U+ qPCR Mix (One Tube)
A 2× multiplex qPCR premix combining antibody-based hot-start Taq technology with dUTP/UDG carryover contamination control for probe-based DNA amplification.
Antibody hot-start Taq
dUTP / UDG
RUO
Product overview
qNTQX® Universal TaqMan Pro U+ qPCR Mix (One Tube), catalog no. LM203026, is a 2× probe-based multiplex qPCR premix built on an antibody-mediated hot-start Taq DNA polymerase. It is supplied ready to use as a single-tube reagent for DNA amplification and includes a dUTP/UDG carryover-contamination control system. The premix is intended for laboratories developing or running multiplex, probe-based qPCR assays on standard real-time PCR instruments, and is supplied for research use only.
Probe-based multiplexing
Develop multiplex assays with your target-specific primers and probes. Validate the panel, fluorophores and reaction conditions for your workflow.
Antibody hot-start technology
Antibody-based hot-start Taq supports specific amplification. The formulation is designed to limit non-specific amplification in multiplex qPCR.
dUTP / UDG protection
A carryover-control system helps reduce contamination from uracil-containing amplification products. It complements appropriate laboratory controls and clean working practices.
Specifications
| Parameter | Value |
|---|---|
| Product format | 2× TaqMan qPCR mix; one-tube premix |
| Detection chemistry | Probe-based fluorescence quantitative PCR |
| Enzyme technology | Antibody-based hot-start Taq |
Pack sizes & ordering
| Catalog No. | Nominal tests | 2× mix volume |
|---|---|---|
| LM203026ES10 | 100 | 1.25 mL |
| LM203026ES20 | 1,000 | 12.5 mL |
| LM203026ES30 | 10,000 | 125 mL |
| LM203026ES40 | 15,000 | 188 mL |
| LM203026ES50 | 20,000 | 250 mL |
One test (T) is a 25 μL reaction using 12.5 μL of 2× mix.
Performance & validation
In the LM203026 stability study, all four multiplex assay groups remained within ±0.5 Ct and 15% fluorescence deviation of the −20°C control under the tested conditions.
4°C · 7 days
Refrigerated treatment, compared with the frozen control
37°C · 7 days
Elevated-temperature treatment, compared with the frozen control
50 freeze–thaw cycles
Repeated freeze–thaw treatment, compared with the frozen control
Product documents
Product FAQs
LM203026 is a probe-based multiplex qPCR premix. Use it with target-specific primers and probes; it is not a SYBR Green master mix. “One Tube” refers to the single 2× qPCR premix. Add your target-specific primers, probes, DNA template and water to prepare the reaction. Primers and probes are not included. If you prepare assay premixes in advance, validate their stability with your specific formulation. This protocol is for DNA amplification and does not include a reverse-transcription step. Direct RNA amplification has not been established here. For RNA workflows, contact SHT to select a reverse-transcription step or a dedicated one-step RT-qPCR product. Yes. LM203026 includes a dUTP/UDG carryover-control system. The standard protocol provides an optional digestion step at 37°C for 5 minutes. Continue to use appropriate negative controls and clean working practices. Store at −25 to −15°C; shelf life is 18 months. The 7-day study at 37°C evaluates stability under test conditions, not routine storage or shipping. Contact SHT for shipping conditions. The LM203026 evaluation compares four multiplex groups with a −20°C control after 4°C and 37°C treatments for 7 days and after 50 freeze–thaw cycles. Three groups are quadruplex and one is triplex. Reported ΔCt stays within ±0.5 and fluorescence deviation within 15%. See the original report and study conditions above. Choose from 100, 1,000, 10,000, 15,000 or 20,000 tests, based on 25 μL reactions. Include your preferred pack size in your quotation request. SHT will confirm pack configuration and current availability. No. LM203026 is for scientific research use only, not for diagnostic or clinical procedures. Validate its suitability for your research assay and sample matrix.Is LM203026 intended for probe-based or SYBR Green qPCR?
What does “One Tube” mean for reaction preparation?
Can this mix amplify RNA directly?
Does the mix include carryover contamination control?
Can the product be stored or shipped at room temperature?
What evidence supports multiplex stability?
Which pack sizes are available?
Is this product for diagnostic use?