Applications / Plant-breeding
Turn plant DNA into confident breeding decisions
Molecular biology gives breeding teams a direct way to measure genetic traits, verify identity and move promising material forward with confidence – from leaf or seed sample to selection decision.
Plant molecular analysis
From leaf or seed sample to selection insight
HOW IT WORKS
From plant sample to a breeding decision
DNA-based analysis supports faster, more objective decisions across breeding programmes. The right workflow protects DNA quality from diverse plant matrices and delivers consistent data for marker, identity and trait analysis.
01 / SAMPLE
Collect leaf or seed
Plant tissue, seeds or protoplasts are prepared for representative, traceable sampling.
02 / EXTRACT
Isolate plant DNA
Magnetic-bead chemistry removes plant-derived inhibitors while recovering clean DNA.
03 / DETECT
Amplify the marker
qPCR assays target the sequence, marker or trait relevant to the breeding question.
04 / SELECT
Act on the result
Reliable data supports selection, identity confirmation and programme decisions.
Application areas
Molecular tools for each stage of breeding
Build dependable workflows for trait selection, variety verification and high-throughput screening.
Marker-assisted selection
Screen for inherited markers that help advance plants with the desired traits.
Variety identity
Confirm cultivar identity and support accurate line management across breeding programmes.
Seed purity
Support purity and hybrid verification with targeted molecular analysis.
Trait screening
Use validated molecular targets to screen breeding material at scale.
Build your workflow
Products for plant breeding
Combine automated extraction, plant-ready reagents, qPCR chemistry and compatible consumables for robust molecular breeding workflows.
01 — DNA / RNA extraction
The bottleneck step, automated and matrix-ready
Extraction quality determines whether the amplification step can run. Medino’s magnetic-bead workflow supports demanding plant matrices on two automated platforms sized to your laboratory’s daily sample volume.
Extraction instruments
S-48 Nucleic Acid Extraction System
Benchtop 48-channel system for satellite laboratories and mid-volume QC lines that still need walk-away extraction.
Cat. S-48
48 channels
N-96 Fully Automated Nucleic Acid Extractor
96-channel walk-away platform for central-lab throughput-a full plate of samples extracted with one protocol and one operator touch.
Cat. N-96
96 channels
Pre-filled extraction kits (magnetic bead-based)
| Product | Cat. No. | Feature | Specification |
|---|---|---|---|
| NUCQX® Universal Magnetic Bead-Based Plant DNA Extraction Kit (Papain) | LM102148 | Pre-filled Extraction Kits (Magnetic Bead-based) | 16 T/96 T (pre-filled) / 20 T/100 T/500 T (bulk) |
| NUCQX® Universal Magnetic Bead-Based Plant DNA Extraction Kit | LM102111 | Pre-filled Extraction Kits (Magnetic Bead-based) | 50 T/100 T/500 T |
02 — qPCR master mix
Five chemistries, one decision: how the sample gets to you
The right master mix depends less on the target than on the logistics around it – whether the reagent needs to survive a warm shipment, run in minutes on a crowded QC line, or resist carryover contamination.
| Product | Cat. No. | Feature | Specification |
|---|---|---|---|
| qNTQX® qPCR SYBR Green Master Mix (No Rox) | LM601003 | Hot-Start SYBR Green I, Melt-Curve Analysis | 5 × 1 ml/100 × 1 ml |
| qNTQX® Universal Superfast qPCR Mix (One Tube) | LM602016 | One-Tube Pre-Mixed | 100 T/1,000 T/10,000 T |
| qNTQX® Universal Multiplex qPCR Master Mix (Probe Based) | LM602002 | Universal (Probe Method) | 1 ml/5×1 ml/50×1 ml |
| qNTQX® Lyo-Ready I TaqMan Multiplex qPCR Mix | LM602011 | A lyophilizable reagent | 100 T/1,000 T |
| qNTQX® Lyo-Ready I TaqMan Multiplex qPCR Mix (Bead in Tubes) | LM602011-C | Lyophilized beads in PCR tubes | 16 T |
| qNTQX® Universal TaqMan Pro U+ qPCR Mix (One Tube) | LM203026 | Stability, dUTP / UDG | 100 T/1,000 T/10,000 T/15,000 T/20,000 T |
03 — NGS
From plant DNA to sequencing-ready libraries
Build your plant sequencing workflow with DNA library preparation, magnetic bead-based cleanup and size selection, and sensitive dsDNA quantification. Choose the reagents that fit your DNA input, target fragment size, and sequencing goals—from crop genomics to breeding research.
| Product | Cat. No. | Feature | Specification |
|---|---|---|---|
| 1× SEQQX® dsDNA HS Assay Kit | LM801001 | Ready-to-use / High sensitivity (0.2–100 ng) / Qubit-compatible | 100/500 T |
| SEQQX® DNA Selection Beads | LM801002 | SPRI-based / DNA size selection / NGS library purification | 1/5/10/60/450 mL |
| SEQQX® OnePot Pro DNA Library Prep Kit | LM801003 | Enzymatic DNA library preparation | 8 T / 24 T / 96 T / 96 T (automation-ready plate) |
04 — Consumables
The small details that keep a food-safety workflow moving.
Reliable molecular results depend on more than chemistry. From sample collection through magnetic-bead separation, compatible consumables protect sample integrity and keep each extraction step clean and ready for throughput.
| Product | Cat. No. | Feature | Specification |
|---|---|---|---|
| ValliQ® Sterile Sampling Bags | ME101055 | collecting, storing, and transporting solid, semi-solid, and liquid samples. Temperature range of 220°C to 240°C. | various sizes |
| ValliQ® 96 Deep-Well Plates | ME10421 | Compatible Consumables for Instruments | 96 wells |
| ValliQ® 96-Well Magnetic Rod Tip Comb | ME10411 | Compatible Consumables for Instruments | 96 wells |
Talk to us
Build a plant-testing workflow around your programme
Contact SHT Technology GmbH to discuss sample type, throughput and suitable molecular workflow components.